引物名稱 引物序列（5’-3')
1492R TACGGCTACCTTGTTACGACTT
27F AGAGTTTGATCCTGGCTCAG
35S GACGCACAATCCCACTATCC
3'AD AGATGGTGCACGATGCACAG
3'AOX(pPIC9K-R) GGCAAATGGCATTCTGACAT
3'BD TAAGAGTCACTTTAAAATTTGTATAC
3Xflag.F GCTCTTGTTGGAGCTGCAGT
3Xflag.R TAATTTTATTAGGACAAGGCTG
5'AOX(pPIC9K-F) GACTGGTTCCAATTGACAAGC
a-Factor TACTATTGCCAGCATTGCTGC
BAC1 AACCATCTCGCAAATAAATA
BAC2 ACGCACAGAATCTAGCGCTT
BGH TAGAAGGCACAGTCGAGG
CMV-24 TTAGGACAAGGCTGGTGG
CMV-30 ATAACCCCGCCCCGTTG
CMV-F CGCAAATGGGCGGTAGGCGTG
DuetDOWN1 GATTATGCGGCCGTGTACAA
DUETUP1 GATCTCGACGCTCTCCCT
DUETUP2 TTGTACACGGCCGCATAATC
EBV-R GTGGTTTGTCCAAACTCATC
Forward TCGcgttaacgctagcatggatctc
Forward(left ITR) CCTCTGACTTGAGCGTCGAT
GFP-F GGTCCTTCTTGAGTTTGTAAC
GFPfusionREV CTCCACTGACAGAAAATTTG
GFP-R CCATCTAATTCAACAAGAATTGGGACAAC
GLP1 TGTATCTTATGGTACTGTAACTG
GLP2 CTTTATGTTTTTGGCGTCTTCC
GLP4 GGGTAGAATGGCGCTGGG
H1 TCGCTATGTGTTCTGGGAAA
hGH-PA-R CCAGCTTGGTTCCCAATAGA
HSVtk-pA-R GTCTCCTTCCGTGTTTCAG
ITS1 TCCGTAGGTGAACCTGCGG
ITS4 TCCTCCGCTTATTGATATGC
M13F（M13-20） TGTAAAACGACGGCCAGT
M13F(-47) CGCCAGGGTTTTCCCAGTCACGAC
M13R CAGGAAACAGCTATGACC
M13R(-48)(即RV-M 引物） AGCGGATAACAATTTCACACAGGA
MalE primer GGTCGTCAGACTGTCGATGAAGCC MBP Tag
MCHERRY-F AACGAGGACTACACCATCGT
mCherry-R TTGGTCACCTTCAGCTTGG
mU6F CAGCACAAAAGGAAACTCACC
NEBF CCCGCCGCTGCTTTTGCACGTGAG
NEBR´ TATGCTAGTTATTGCTCAG
NOS GATAATCATCGCAAGACCGG
P1 CCAGGCTTTACACTTTATGC
P10 GCCTCTAACATTGAGACAGC
P17110 TACCACTACAATGGATG
P2 GCGATTAAGTTGGGTAACGC
P20 AAGAGTTACTCAAGAACAAGAA
P24990 TCATCGGAAGAGAGTAG
P3' CTGCAAGGCGATTAAGTTGG
P5' TGCGTACTGCGGTGATCAAC
PAAV-MCS-F TCATACCTCTTATCTTCCTC
PABAI-R ATGCTACAAAGGACCTAATG
pACT2.R GTGCACGATGCACAGTTGAA
GTGCACGATGCACAGTTGAA
PAO-AAM.F GACTGGTTCCAATTGACAAG
pAS2-1.R AAGAGTTACTCAAGAACAAGAA
pB42ADF CCAGCCTCTTGCTGAGTGGAGATG
pB42ADR AAGCCGACAACCTTGATTGGAG
pBABE-3 accctaactgacacacattcc SV40 promoter(SV40 ori)測(cè)啟動(dòng)子相反方向
pBABE-5 ctttatccagccctcac gag (truncated)
PBAD30.F AGATTAGCGGATCCTACCTG araBAD promoter
PBAD
