中文名稱 | 核因子2相關(guān)因子2抗體 |
別 名 | Nuclear factor-E2 related factor2; Nrf-2; HEBP1; NF E2 related factor 2; NFE2 related factor 2; NFE2L2; Nrf 2; Nuclear factor erythroid 2 related factor 2; Nuclear factor erythroid derived 2 like 2; NF2L2_HUMAN. |
研究領域 | 腫瘤 免疫學 染色質(zhì)和核信號 合成與降解 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Human, Mouse, Rat, (predicted: Chicken, Dog, Cow, Horse, Rabbit, ) |
產(chǎn)品應用 | WB=1:100-1000 ELISA=1:1000-5000 IHC-P=1:100-1000 IHC-F=1:100-1000 ICC=1:100 IF=1:100-1000 (石蠟切片需做抗原修復) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 68kDa |
細胞定位 | 細胞核 細胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human Nrf2:401-500/605 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
PubMed | PubMed |
產(chǎn)品介紹 | Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Function: Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Subunit: Heterodimer. May bind DNA with an unknown protein. Interacts with KEAP1. Interacts via its leucine-zipper domain with the coiled-coil domain of PMF1. Subcellular Location: Cytoplasm, cytosol. Nucleus. Note=Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents. Tissue Specificity: Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle. Post-translational modifications: Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus. Similarity: Belongs to the bZIP family. CNC subfamily. Contains 1 bZIP domain. SWISS: Q16236 Gene ID: 4780 Database links: Entrez Gene: 4780 Human Omim: 600492 Human SwissProt: Q16236 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 轉(zhuǎn)錄調(diào)節(jié)因子(Transcriptin Regulators) 核細胞系因子2相關(guān)因子Nrf2,是一種對氧化還原反應敏感的轉(zhuǎn)錄因子,可以通過正向促進基因的表達,來轉(zhuǎn)錄翻譯產(chǎn)生一些抗氧化物,行使著異化解毒的酶類和藥物的高效外排泵作用。 有學者認為:核因子2相關(guān)因子2蛋白在保護細胞免受環(huán)境毒性物質(zhì)影響方面發(fā)揮著極其重要的作用,它指導某些基因促進細胞的防御工事,同時也刺激某些重要解毒酶對毒性物質(zhì),如黃曲霉素的解毒和排毒作用。 |
產(chǎn)品圖片 | Sample: Lane 1: Kidney (Mouse) Lysate at 40 ug Lane 2: Liver (Mouse) Lysate at 40 ug Lane 3: Liver ((Rat) Lysate at 40 ug Lane 4: SiHa (Human) Cell Lysate at 30 ug Lane 5: HepG2 (Human) Cell Lysate at 30 ug Primary: Anti-Nrf2 (bs-1074R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 110’68 kD Observed band size: 110 kD Sample: HepG2(Human) Cell Lysate at 40 ug MCF-7(Human) Cell Lysate at 40 ug Primary: Anti-Nrf2(bs-1074R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 68kD Observed band size: 68kD Sample:A549 (Human)Cell Lysate at 40 ug Primary: Anti-Nrf2(bs-1074R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 68kD Observed band size: 68kD Sample: Brain(Rat) lysate at 30ug; Brain(Mouse) lysate at 30ug; Primary: Anti-Nrf2 (bs-1074R) at 1:200 dilution Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 dilution Predicted band size : 66kD Observed band size : 66kD Primary: Anti-Nrf2 (bs-1074R) at 1:200 Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 Predicted band size : 66kD Observed band size : 66kD Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (bs-1074R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.Tissue/cell: human endometrium carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Nrf2 Polyclonal Antibody, Unconjugated(bs-1074R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Nrf2)) polyclonal Antibody, Unconjugated (bs-1074R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. |
我要詢價
*聯(lián)系方式:
(可以是QQ、MSN、電子郵箱、電話等,您的聯(lián)系方式不會被公開)
*內(nèi)容: