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中文名稱

CD4抗體

別名

CD4 (L3T4); CD4 antigen (p55); CD4 Antigen ; CD4 molecule; CD4 Receptor; CD4+ Lymphocyte deficiency, included; CD4mut; L3T4; Leu3; Ly-4; Lymphocyte antigen CD4; MGC165891; p55; T Cell Antigen T4 ; T cell antigen T4/LEU3; T cell differentiation antigen L3T4; T cell OKT4 deficiency, included; T cell surface antigen T4/Leu 3 ; T cell surface antigen T4/Leu3; T Cell Surface Glycoprotein CD4; W3/25; W3/25 antigen; T-cell surface glycoprotein CD4 isoform 1 precursor; CD4_HUMAN.

研究領(lǐng)域	腫瘤細(xì)胞生物免疫學(xué) 干細(xì)胞轉(zhuǎn)錄調(diào)節(jié)因子細(xì)胞表面分子 t-淋巴細(xì)胞
抗體來(lái)源	Rabbit
克隆類型	Polyclonal
交叉反應(yīng)	Human, Mouse, Rat, Pig, MACFA (predicted: Dog, Cow, Sheep, Guinea Pig, )
產(chǎn)品應(yīng)用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	48kDa
細(xì)胞定位	細(xì)胞膜
性狀	Liquid
濃度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human CD4:385-457/457
亞型	IgG
純化方法	affinity purified by Protein A
儲(chǔ) 存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed	PubMed
產(chǎn)品介紹	This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]. Function: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Subunit: Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI-CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Note=Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. Post-translational modifications: Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. Similarity: Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: P01730 Gene ID: 920 Database links: Entrez Gene: 920 Human Entrez Gene: 12504 Mouse Omim: 186940 Human SwissProt: P06332 Mouse SwissProt: P01730 Human Unigene: 631659 Human Unigene: 2209 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 此抗體可識(shí)別55KDⅠ型單鏈穿膜糖蛋白。 CD4分子是存在于大多數(shù)輔助/誘導(dǎo)T細(xì)胞表面的59kDa的糖蛋白。正常淋巴組織中CD4的表達(dá)數(shù)量多于CD8，此抗體主要用于標(biāo)記輔助/誘導(dǎo)T細(xì)胞，與CD8單抗聯(lián)合使用對(duì)外周血淋巴細(xì)胞分型。 CD4抗原是HLA-II類分子和人類免疫缺陷病毒(HIV)-愛(ài)滋病的受體，在35-50%外周血淋巴細(xì)胞-輔助和誘導(dǎo)T細(xì)胞(Th/Ti)和70-80%人胸腺細(xì)胞上表達(dá)，在人的單核細(xì)胞表面也有低密度的表達(dá)。 CD4抗原有膜結(jié)合型和可溶性兩種形式。Th/Ti可輔助Ig產(chǎn)生和T細(xì)胞毒T細(xì)胞的作用。
產(chǎn)品圖片	Sample: HL60 Cell Lysate at 40 ug Primary: Anti- CD4 (bs-0647R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 55kD U937 Cell Lysate at 40 ug Primary: Anti- CD4 (bs-0647R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 55kD Sample: Lane 1: U937 (Human) Cell Lysate at 30 ug Lane 2: HL-60 (Human) Cell Lysate at 30 ug Lane 3: Jurkat (Human) Cell Lysate at 30 ug Primary: Anti-CD4 (bs-0647R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti- CD4 (bs-0647R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 55 kD Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti- CD4 (bs-0647R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 55 kD Sample: HUVEC(Human) Cell Lysate at 30 ug Primary: Anti- CD4 (bs-0647R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 55 kD Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti- CD4 (bs-0647R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 53 kD Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti- CD4 (bs-0647R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 55kD Sample: Lymph node(Mouse) Lysate at 40 ug Primary: Anti- CD4 (bs-0647R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 55kD Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD4) Polyclonal Antibody, Unconjugated (bs-0647R) at 1:4000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD4) Polyclonal Antibody, Unconjugated (bs-0647R) at 1:4000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Image was kindly submitted by Dr.David M Burmeister from US Army Institute of Surgical Research. Pig lymph nodes stained with Rabbit Anti-CD4 Polyclonal Antibody(bs-bs-0647R)at 1:300 for one hour at room temperature.Blank control (blue line): HL60 (fixed with 2% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-CD4 antibody (bs-0647R),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,dilution: 1μg /test.