研究領(lǐng)域 細(xì)胞生物 免疫學(xué) 細(xì)胞表面分子 糖蛋白 淋巴細(xì)胞 t-淋巴細(xì)胞
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human, (predicted: Mouse, Rat, )
產(chǎn)品應(yīng)用 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 28kDa
細(xì)胞定位 細(xì)胞膜
性 狀 Liquid
濃 度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CD200:41-140/278
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
產(chǎn)品介紹 This gene encodes a type I membrane glycoprotein containing two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. This gene is expressed by various cell types, including B cells, a subset of T cells, thymocytes, endothelial cells, and neurons. The encoded protein plays an important role in immunosuppression and regulation of anti-tumor activity. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]
Function:
Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.
Subunit:
Interacts with CD200R1.
Subcellular Location:
Cell membrane; Single-pass type I membrane protein.
Similarity:
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
SWISS:
P41217
Gene ID:
4345
Database links:
Entrez Gene: 4345 HumanOmim: 155970 HumanSwissProt: P41217 HumanUnigene: 79015 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
CD200是Ⅰ型膜糖蛋白，為免疫球蛋白超家族的新成員，廣泛表達(dá)在人類胸腺細(xì)胞、B細(xì)胞、激活的T細(xì)胞、濾泡狀樹(shù)突細(xì)胞、神經(jīng)元及血管內(nèi)皮上。可表達(dá)于B細(xì)胞及其增殖性疾病和一部分T細(xì)胞。近年來(lái)研究發(fā)現(xiàn)，CD200與CD200R間的相互作用可以傳遞抑制性信號(hào)減低髓樣細(xì)胞的活性，改變其遷移。CD200在免疫疾病和抑制排斥反應(yīng)中作為一種免疫耐受信號(hào)，它的一些相關(guān)分子在免疫調(diào)節(jié)中也具有一定的作用。
| 產(chǎn)品圖片 |  Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD200) Polyclonal Antibody, Unconjugated (Ys-6030R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (Human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD200) Polyclonal Antibody, Unconjugated (Ys-6030R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Tissue/cell: human rectal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CD200 Polyclonal Antibody, Unconjugated(Ys-6030R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CD200 Polyclonal Antibody, Unconjugated(Ys-6030R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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