| 中文名稱 | 凋亡研究抗體套裝 |
| 別 名 | Apoptosis Research Antibody Assembly kit(Bax,Caspase-3,Caspase-8,Caspase-9,Cytochrome C) |
| 研究領(lǐng)域 | 細(xì)胞生物 細(xì)胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, Rat, |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | : |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | Apoptosis is the most common type of programmed cell death. The signal transduction of apoptosis involves the processing and activation of cysteinase caspase and the alternative mitochondrial pathway mediated by pro-apoptosis factors. Among them, the caspase mediating the initiation of apoptosis includes caspase-8,-9,-10 and -12, in which caspase-8 is mainly involved in tumor necrosis factor receptor (TNFR) superfamily death receptor mediated extrinsic apoptosis pathway. Caspase-3,-6 and -7 mediate downstream effects, including cleavage of cytoskeleton and chromatin, and eventually lead to apoptosis. In addition, mitochondria also play key roles in activation of apoptosis. Different upstream stimuli promote the binding of pro-apoptosis factors to the outer membrane of mitochondria through signal transduction, which leads to the change of mitochondrial permeability and the release of inner mitochondrial membrane (IMM) proteins (such as cytochrome C and other proteins). Cytochrome C coupling activation of caspase-9 can further activate key downstream effector caspases. Apoptosis Research Antibody Assembly Kit is one economical package of selected antibodies for most representative apoptosis signaling pathway-related proteins, including caspase-8, Bax, cytochrome C, caspase-9 and caspase-3. These antibodies have been tested in a variety of applications and species. For specific application information of different antibodies, please refer to each individual antibody datasheet. Apoptosis Research Antibody Assembly kit Mouse Anti-Bax antibody (bsm-33279M) Rabbit Anti-Caspase 3 precursor antibody (bs-2593R) Rabbit Anti-Caspase-8 subunit p10 antibody (bs-20613R) Rabbit Anti-Caspase-9 antibody (bs-20773R) Rabbit Anti-Cytochrome C antibody (bsm-52050R) Database links: Apoptosis Research Antibody Assembly kit Mouse Anti-Bax antibody (bsm-33279M) Rabbit Anti-Caspase 3 precursor antibody (bs-2593R) Rabbit Anti-Caspase-8 subunit p10 antibody (bs-20613R) Rabbit Anti-Caspase-9 antibody (bs-20773R) Rabbit Anti-Cytochrome C antibody (bsm-52050R) Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 細(xì)胞凋亡是一種最常見的細(xì)胞程序性死亡。凋亡的信號傳導(dǎo)過程涉及半胱氨酸蛋白酶caspase 的加工和激活過程以及由促凋亡因子介導(dǎo)的線粒體旁路途徑。其中，介導(dǎo)凋亡起始的caspase包括caspase-8、-9、-10 和 -12，其中caspase-8主要參與由腫瘤壞死因子受體（TNFR）超家族死亡受體介導(dǎo)的外源性凋亡途徑。而介導(dǎo)下游效應(yīng)的 caspase 包括 caspase-3、-6 和 -7，這些下游效應(yīng)caspase 會導(dǎo)致細(xì)胞骨架蛋白和染色質(zhì)被剪切并最終發(fā)生細(xì)胞凋亡。另外，凋亡還可以通過線粒體旁路途徑發(fā)生，一些不同的上游應(yīng)激通過信號傳導(dǎo)過程促進(jìn)促凋亡因子結(jié)合于線粒體外膜，導(dǎo)致線粒體通透性改變并釋放線粒體內(nèi)膜蛋白（如細(xì)胞色素C等蛋白）。細(xì)胞色素C偶聯(lián)激活的 caspase-9，會進(jìn)一步激活下游關(guān)鍵效應(yīng)的 caspase并最終發(fā)生細(xì)胞凋亡。 凋亡研究抗體套裝極具性價(jià)比，包含了最具代表性的凋亡信號通路相關(guān)蛋白，包括介導(dǎo)外源性凋亡途徑的caspase-8、介導(dǎo)線粒體旁路途徑的促凋亡因子Bax和線粒體內(nèi)膜蛋白細(xì)胞色素C、參與多條凋亡分支途徑的caspase-9以及介導(dǎo)下游效應(yīng)的caspase-3。這些抗體在多種應(yīng)用和物種中進(jìn)行了測試。有關(guān)抗體具體使用信息，請參閱各個(gè)抗體產(chǎn)品數(shù)據(jù)表。 |
| 產(chǎn)品圖片 |  Primary: Anti-Caspase-9 (bs-20773R) at 1/300 dilutionSample: K562 Cell (Human) Lysate at 40 ug Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35/50 kD Observed band size: 35 kD  Primary: Anti-Caspase 3 precursor (bs-2593R) at 1/1000 dilutionSample: Spleen (Mouse) Lysate at 40 ug Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28 kD  Primary: Anti-Bax (bsm-33279M) at 1/1000 dilutionSample: 293T(Human) Cell Lysate at 30 ug 293T-UV(Human) Cell Lysate at 30 ug Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 21 kD  Primary: Anti- Cytochrome C (bsm-52050R) at 1/1000 dilutionSample: Liver (Mouse) Lysate at 40 ug Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12.8/26 kD Observed band size: 12.8 kD  Primary: Anti- Caspase 8 (bs-20613R) at 1/300 dilutionSample: Thymus (Mouse) Lysate at 40 ug Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12,55 kD Observed band size: 17 kD  Primary: Anti- Caspase 8 (bs-20613R)Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase 8) Polyclonal Antibody, Unconjugated (bs-20613R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.  Primary: Anti- Cytochrome C (bsm-52050R)Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome C) Polyclonal Antibody, Unconjugated (bsm-52050R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  primary: Anti-Caspase-9 (bs-20773R)Paraformaldehyde-fixed, paraffin embedded (Rat urinary bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase-9) Polyclonal Antibody, Unconjugated (bs-20773R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
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