Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Full length recombinant mouse Galectin-9 (M-type)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-F - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 5.0 - 10 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Clone 108A2 recognizes the linker peptide of mouse Galectin-9; it does not cross-react with stable Galectin-9 (which is biologically active, genetically engineered, linker-less Galectin-9)3. Additional reported applications (for the relevant formats) include: sandwich ELISA as capture antibody1 and immunohistochemical staining of paraffin2 embedded tissue sections.
- Application References
(PubMed link indicates BioLegend citation) - Product Citations
-
- RRID
- AB_2728158 (BioLegend Cat. No. 137907) AB_2728159 (BioLegend Cat. No. 137908)
Antigen Details
- Structure
- Homodimer, each chain is a 343 aa protein of 40 kD that has two conserved carbohydrate recognition domains (CRDs)
- Distribution
-
Lymphocytes, dendritic cells, neutrophils, eosinophils, astrocytes, endothelial cells, fibroblasts, thymus stromal/epithelial cells
- Function
- Cell aggregation, cell adhesion, chemotaxis, apoptosis, suppression of Th1 and Th17 responses, induction of regulatory T cells
- Ligand/Receptor
- Tim-3
- Cell Type
- Astrocytes, Dendritic cells, Endothelial cells, Eosinophils, Epithelial cells, Fibroblasts, Lymphocytes, Neutrophils, Tregs
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
- Molecular Family
- Adhesion Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Klibi J, et al. 2009. Blood 113:1957.
2. Seki M, et al. 2008. Clin. Immunol. 127:78.
3. Tsuboi Y, et al. 2007. Clin. Immunol. 124:221.
4. Zhu C, et al. 2005. Nat. Immunol. 6:1245.
5. Dunphy JL, et al. 2002. J. Biol. Chem. 277:14916. - Gene ID
- 16859 View all products for this Gene ID
- UniProt
- View information about Galectin-9 on UniProt.org
