Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
IHC-P - Verified - Recommended Usage
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/ml is recommended. For immunohistochemical staining on?formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses- Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemistry1.
- Application References
(PubMed link indicates BioLegend citation) -
- Jozef S, et al. 2012. Biomed. Pap. Med. Fac. 156:135. (IHC)
- Product Citations
-
- RRID
- AB_2616772 (BioLegend Cat. No. 656810)
Antigen Details
- Structure
- 1621 amino acids with predicted molecular weight of 200-220 kD
- Distribution
-
Cytosol
- Function
- Required for survival and renewal of neural progenitor cells; plays roles in angiogenesis and tumor formation
- Interaction
- Vimentin, alpha-internexin and FHOD3
- Cell Type
- Neural Stem Cells
- Biology Area
- Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- Intermediate Filaments
- Antigen References
-
1. Matsuda Y, et al. 2013. World J. Gastroenterol. 19:42.
2. Matsuda Y, et al. 2012. Med. Mol. Morphol. 45:59.
3. Krupkova O Jr, et al. 2010. Neoplasma. 57:291.
4. Hombach-Klonisch S, et al. 2008. Arch. Immunol. Ther. Exp. (Warsz). 56:165.
5. Pilkington GJ, et al. 2005. Cell Prolif. 38:423. - Gene ID
- 10763 View all products for this Gene ID
- UniProt
- View information about Nestin on UniProt.org
