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Brilliant Violet 421™ anti-mouse CD274 (B7-H

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Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence4, blocking6,7,8,9, and immunohistochemistry of acetone-fixed frozen sections4, 11. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 124303). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 124318) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).

Application References

(PubMed link indicates BioLegend citation)
  1. Maier H, et al. 2007. J. Immunol. 178:2714.
  2. Meng Q, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:4444. PubMed
  3. Scarlett UK, et al. 2012. J Exp Med. 209:495. PubMed
  4. Grabie N, et al. 2007. Circulation 116:2062. (IF, IHC)
  5. Paterson AM, et al. 2011. J. Immunol. 187:1097.
  6. Channappanavar R, et al. 2012. PLoS One 7:e39757. (Block)
  7. Schreiber HA, et al. 2010. PLoS One 5:e11453. (Block) PubMed
  8. Muthumani K, et al. 2011. J. Immunol. 187:2932. (Block) PubMed
  9. Cripps JG, et al. 2010. Hepatology 52:1350. (Block) PubMed
  10. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  11. Riella LV, et al. 2011. Am. J. Transplant 11:832-40. (IHC)
  12. Lei GS, et al. 2015. Infect Immun. 83:572. PubMed
Product Citations
  1. Yao RQ, et al. 2022. Theranostics. 12:4606. PubMed
  2. Saha D et al. 2017. Cancer cell. 32(2):253-267 . PubMed
  3. Wei H, et al. 2021. Malar J. 20:89. PubMed
  4. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  5. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
  6. Hsu JM, et al. 2018. Nat Commun. 9:1908. PubMed
  7. Stewart JM, et al. 2020. ACS Biomater Sci Eng. 6:5941. PubMed
  8. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  9. Giesen D, et al. 2020. Clin Cancer Res. 26:3999. PubMed
  10. Puigdelloses M, et al. 2021. J Immunother Cancer. 9:. PubMed
  11. Walsh SM, et al. 2021. eLife. 10:00. PubMed
  12. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  13. Yu D, et al. 2019. Cell Commun Signal. 0.828472222. PubMed
  14. Jiao S, et al. 2017. Clin Cancer Res. 23:3711. PubMed
  15. Zhao Y, et al. 2020. Immunity. 51(6):1059-1073.e9.. PubMed
  16. Vogel A, et al. 2022. Cell Rep. 38:110420. PubMed
  17. Ng KW, et al. 2019. eLife. 0.333333333333333. PubMed
  18. van der Velden V, et al. 2017. J Immunol Methods. 10.1016/j.jim.2017.03.011. PubMed
  19. Rosato PC, et al. 2019. Nat Commun. 10:567. PubMed
  20. Sugita Y, et al. 2021. Oncol Rep. 45:. PubMed
  21. Bommareddy PK, et al. 2019. J Biol Methods. 6:2. PubMed
  22. Freed-Pastor WA, et al. 2021. Cancer Cell. :. PubMed
  23. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  24. O'Reilly LA, et al. 2018. Immunity. 48:570. PubMed
  25. Lucas ED, et al. 2020. Cell Reports. 33(2):108258. PubMed
RRID
AB_10897097 (BioLegend Cat. No. 124315)

Antigen Details

Structure
40 kD type I transmembrane protein member of B7 family within the immunoglobulin receptor superfamily
Distribution

T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes

Ligand/Receptor
PD-1 (PDCD1)
Cell Type
B cells, Dendritic cells, Endothelial cells, Monocytes, NK cells, T cells
Biology Area
Cancer Biomarkers, Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Sharpe A, et al. 2007. Nat. Immunol. 8:239.
2. Dong H, et al. 1999. Nat. Med. 5:1365.
3. Freeman G, et al. 2000. J. Exp. Med. 192:1027.

Gene ID
60533 View all products for this Gene ID
UniProt
View information about CD274 on UniProt.org
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