Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- CKB mouse splenocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, blocking of MHC class II - restricted CD4+ T cell responses2,3, induction of B cell differentiation4, and immunohistochemical staining5 of acetone-fixed frozen sections.
This clone does not cross react with other haplotypes (e.g., b, d, f, p, q, s).- Application References
(PubMed link indicates BioLegend citation) -
- Oi VT, et al. 1978. Curr. Top. Microbiol. Immunol. 81:115. (IP)
- Formby B, et al. 1990. P. Natl. Acad. Sci. USA 87:2438.
- Kappler JW, et al. 1981. J. Exp. Med. 153:1198.
- Bishop GA, et al. 1989. P. Natl. Acad. Sci. USA 86:5933.
- Ardehali A, et al. 1995. Circulation 92:450. (IHC)
- Turnquist HR, et al. 2007. J. Immunol. 178:7018.
- Product Citations
-
- RRID
- AB_313463 (BioLegend Cat. No. 110006)
Antigen Details
- Structure
- α chain of MHC class II
- Distribution
-
B cell and activated T cells, APCs of H-2b mice
- Function
- Antigen presentation
- Ligand/Receptor
- CD3/TCR, CD4
- Cell Type
- Antigen-presenting cells, B cells, T cells, Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
-
1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. - Gene ID
- 14960 View all products for this Gene ID
- UniProt
- View information about I-Ak on UniProt.org
