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中文名稱

過(guò)氧化酶活化增生受體γ抗體

別名

CIMT1; HUMPPARG; NR1C3; Nuclear receptor subfamily 1 group C member 3; PAX8/PPARG Fusion Gene; Peroxisome Proliferator Activated Receptor gamma; PPAR gamma; PPARG; PPARG1; PPARG2; PPARG3; CIMT1; GLM1; HUMPPARG; NR1C3; Nuclear receptor subfamily 1 group C member 3; PAX8/PPARG Fusion Gene; Peroxisome proliferator activated nuclear receptor gamma variant 1; Peroxisome proliferator activated receptor gamma 1; Peroxisome Proliferator Activated Receptor gamma; Peroxisome proliferator-activated receptor gamma; PPAR gamma; PPAR-gamma; PPARG_HUMAN; PPAR gamma 1; PPAR gamma 2; PPAR gamma 3; PPAR gamma-1; PPAR gamma-2; PPAR gamma-3.

研究領(lǐng)域	免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子激酶和磷酸酶糖尿病內(nèi)分泌病
抗體來(lái)源	Rabbit
克隆類型	Polyclonal
交叉反應(yīng)	Human, Mouse, Rat, (predicted: Chicken, Pig, Cow, Rabbit, Sheep, )
產(chǎn)品應(yīng)用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	57kDa
細(xì)胞定位	細(xì)胞核
性狀	Liquid
濃度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human PPAR Gamma:101-200/505
亞型	IgG
純化方法	affinity purified by Protein A
儲(chǔ) 存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed	PubMed
產(chǎn)品介紹	The PPAR gamma antibody mainly is exist in the white fat organization, the fat for the PPAR gamma is born, blood sugar stability, the disease respond, the artery gruel kind hardens to rise the important function with the tumor occurrence of etc. all, but concerning the PPAR gamma to bone of function is a new research heat to order in recent years.A PPAR of many researches report gamma was go together with the body is after activate can the function promote many capable cells divided to increase to living but repress the ossification cell to divide to cause the bone measure the decrease or bone softs toward the fat cell in the marrow, the PPAR gamma promotes the ability and bones that the fat cell divide metabolize closely related, the performance is increasing along with the growth marrow fat content of the age, the ossification cell metabolism the outcome reduce, the different construction PPAR gamma 2 have the important function. Function: Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. Subunit: Forms a heterodimer with the retinoic acid receptor RXRA called adipocyte-specific transcription factor ARF6. Interacts with NCOA6 coactivator, leading to a strong increase in transcription of target genes. Interacts with coactivator PPARBP, leading to a mild increase in transcription of target genes. Interacts with FAM120B. Interacts with PRDM16 (By similarity). Interacts with NOCA7 in a ligand-inducible manner. Interacts with NCOA1 LXXLL motifs. Interacts with TGFB1I1. Interacts with DNTTIP2. Interacts with PRMT2. Subcellular Location: Nucleus. Tissue Specificity: Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary. DISEASE: Note=Defects in PPARG can lead to type 2 insulin-resistant diabetes and hyptertension. PPARG mutations may be associated with colon cancer. Defects in PPARG may be associated with susceptibility to obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) [MIM:604367]. Familial partial lipodystrophies (FPLD) are a heterogeneous group of genetic disorders characterized by marked loss of subcutaneous (sc) fat from the extremities. Affected individuals show an increased preponderance of insulin resistance, diabetes mellitus and dyslipidemia. Genetic variations in PPARG can be associated with susceptibility to glioma type 1 (GLM1) [MIM:137800]. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Note=Polymorphic PPARG alleles have been found to be significantly over-represented among a cohort of American patients with sporadic glioblastoma multiforme suggesting a possible contribution to disease susceptibility. Similarity: Belongs to the nuclear hormone receptor family. NR1 subfamily. Contains 1 nuclear receptor DNA-binding domain. SWISS: P37231 Gene ID: 5468 Database links: Entrez Gene: 5468 Human Entrez Gene: 19016 Mouse Entrez Gene: 25664 Rat SwissProt: P37231 Human SwissProt: P37238 Mouse SwissProt: O88275 Rat Unigene: 162646 Human Unigene: 3020 Mouse Unigene: 23443 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 類固醇受體（Steroid Receptors）過(guò)氧化物酶體增殖物激活受體γ（PPARγ）主要存在于白色脂肪組織，PPARγ對(duì)于脂肪生成、血糖穩(wěn)定、炎癥反應(yīng)、動(dòng)脈粥樣硬化和腫瘤等的發(fā)生都起到重要的作用。主要在脂肪細(xì)胞內(nèi)表達(dá)。PPARγ是噻唑烷二酮類藥物（TZDs）作用的藥靶，又是脂肪細(xì)胞分化的重要調(diào)節(jié)因子。經(jīng)研究發(fā)現(xiàn)，PPARγ在肥胖及胰島素抵抗的發(fā)病機(jī)制中具有十分重要的意義，是治療糖尿病、肥胖等代謝性疾病的重要藥靶。過(guò)氧化物酶體增殖物激活受體γ（PPARγ）屬Ⅱ型核受體超家族成員，主要在脂肪細(xì)胞內(nèi)表達(dá)。PPARγ是噻唑烷二酮類藥物（TZDs）作用的藥靶，又是脂肪細(xì)胞分化的重要調(diào)節(jié)因子�，F(xiàn)有研究（包括一次于美國(guó)加州大學(xué)進(jìn)行的研究）發(fā)現(xiàn)PPARγ在肥胖及胰島素抵抗的發(fā)病機(jī)制中具有十分重要的意義，是治療糖尿病、肥胖等代謝性疾病的重要藥靶。目前，該受體蛋白質(zhì)水平的篩選模式已經(jīng)建立，并正在建立該受體的報(bào)告基因的細(xì)胞水平篩選評(píng)價(jià)模式。
產(chǎn)品圖片	Sample: Heart (Mouse) Lysate at 30 ug Primary: Anti- PPAR gamma (bs-0530R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 51 kD Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug Lane 2: Adipocyte (Rat) Lysate at 40 ug Lane 3: Large intestine (Mouse) Lysate at 40 ug Lane 4: Lung (Mouse) Lysate at 40 ug Primary: Anti-PPAR gamma (bs-0530R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD Sample: Liver (Mouse) Lysate at 40 ug Heart (Mouse) Lysate at 40 ug Lung (Mouse) Lysate at 40 ug Primary: Anti- PPAR gamma (bs-0530R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 51 kD Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-PPAR gamma (bs-0530R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD Sample: 293T(Human) Cell Lysate at 30 ug Primary: Anti-PPAR gamma (bs-0530R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD Sample: Stomach (Mouse) Lysate at 30 ug Primary: Rabbit Anti- PPAR gamma (bs-0530R) at 1:300 dilution; Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size:57 kD Observed band size:55 kDParaformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPAR gamma) Polyclonal Antibody, Unconjugated (bs-0530R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PPAR Gamma Polyclonal Antibody, Unconjugated(bs-0530R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (PPAR gamma) polyclonal Antibody, Unconjugated (bs-0530R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.Blank control (blue line): U251 Primary Antibody (green line): Rabbit Anti-PPARG/PPAR gamma antibody (bs-0530R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.