Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- CRTAM extracellular region-human IgG Fc fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.?
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (for relevant formats) include: Western blotting1.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Arase N, et al. 2005. Int. Immunol. 17:1227. (WB)
- RRID
- AB_2721721 (BioLegend Cat. No. 142009) AB_2721722 (BioLegend Cat. No. 142010)
Antigen Details
- Structure
- 75 kD homodimer, nectin family, Ig superfamily
- Distribution
-
Activated NK cells and activated CD8-positive T cells
- Function
- Cell adhesion, migration
- Ligand/Receptor
- Nectin-like molecule 2
- Cell Type
- NK cells, T cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Signal Transduction
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Takeuchi A, et al. 2009. J. Immunol. 183:4220.
2. Yeh JH, et al. 2008. Cell 132:846.
3. Arase N. 2005. Int. Immunol. 17:1227.
4. Boles KS, et al. 2005. Blood 106:779.
5. Galibert L, et al. 2005 J. Biol. Chem. 280:21955.
6. Kennedy J, et al. 2000 J. Leukoc. Biol. 67:725. - Gene ID
- 54698 View all products for this Gene ID
- UniProt
- View information about CD355 on UniProt.org
